Blog Post Week 10 Gram Stain Method & Robotics
November 7, 2019
This week I used an inoculating loop to transfer my unknown bacteria onto a glass side. I was task to perform the grain stain method. I smear my bacteria with crystal violet, Gram's iodine, Decolorizer, and Safranin. I also made sure to rinse with water. After the process I used a microscope for the first time, and I learn how to use the controls to identify my unknown bacteria. It wasn't easy at first since I needed to adjust the microscope for the right magnification. I discovered that my unknown bacteria was Rods (Bacillus). I got a Gram negative bacilli for my bacteria. The next step was to do the Gram Stain method in which I only did 3 tests. The Oxidase, Glucose Fermentation, and SIM test. My negative bacilli was negative for the Oxidase test. I got positive for the glucose fermentation. On the SIM test I got a negative result which was Escherichia cali.
On Thursday, during the STEM event I helped Josh setting up and made sure the robots were safe. It was fun for me because I get to play with the cool robots and learn something new about robotics. Josh even gave me a cool t-shirt that really amazed me from the details of a bear with robotic arms and legs.
This week I used an inoculating loop to transfer my unknown bacteria onto a glass side. I was task to perform the grain stain method. I smear my bacteria with crystal violet, Gram's iodine, Decolorizer, and Safranin. I also made sure to rinse with water. After the process I used a microscope for the first time, and I learn how to use the controls to identify my unknown bacteria. It wasn't easy at first since I needed to adjust the microscope for the right magnification. I discovered that my unknown bacteria was Rods (Bacillus). I got a Gram negative bacilli for my bacteria. The next step was to do the Gram Stain method in which I only did 3 tests. The Oxidase, Glucose Fermentation, and SIM test. My negative bacilli was negative for the Oxidase test. I got positive for the glucose fermentation. On the SIM test I got a negative result which was Escherichia cali.
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